Method of increasing efficiency of directed evolution of a gene using phagemid
US6156509A · kind A · utility
67Cited by
3References
15Claims
0Family size
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Key dates
| Filing date | Nov 12, 1997 |
| Grant date | Dec 5, 2000 |
| Priority date | — |
| Expiry date | Nov 12, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/102
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method of mutanting DNA is provided comprising mutating a gene bearing phagemid in a host and infecting the host cell with a suitable helper phage to initiate packaging of the DNA into a phagemid. The packaged phagemid is then used to infect a second host cell. The method facilitates more efficient screening for genetic diversity. Also, the gene of interest is mutated and separable from the host cell which undergoes the mutational event reducing false positive results or other artifacts of general mutation techniques.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.