Characterizing polypeptides
US6156527A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 10, 1999 |
| Grant date | Dec 5, 2000 |
| Priority date | — |
| Expiry date | Sep 10, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for characterizing polypeptides, which comprises: (a) treating a sample comprising a population of one or more polypeptides with a cleavage agent which is known to recognize a specific amino acid residue or sequence in polypeptide chains and to cleave at a cleavage site, whereby the population is cleaved to generate peptide fragments; (b) isolating a population of the peptide fragments which bear at one end a reference terminus comprising either only a C-terminus or only an N-terminus and which bear at the other end the cleavage site proximal to the reference terminus; and c) determining a signature sequence of at least some of the isolated fragments, which signature sequence is the sequence of a predetermined number of amino acid residues running from the cleavage site; wherein the signature sequence and the relative position of the cleavage site to the reference terminus characterize the polypeptide or each polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.