Immobilization of enzymes with a fluidized bed for use in an organic medium
US6156548A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 11, 1998 |
| Grant date | Dec 5, 2000 |
| Priority date | — |
| Expiry date | Dec 11, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An immobilized enzyme preparation for use in an organic medium essentially devoid of free water is prepared using a fluidized bed. An enzyme-containing liquid medium is contacted with a particulate porous carrier which preferably has a particle size of 200-1000 .mu.m and a surface area of 20-1000 m.sup.2 /g, and volatile components of the liquid medium are removed to fix or adsorb the enzyme on the carrier. The carrier may have a hydrophilic surface and an amount of liquid medium is used to prevent agglomeration of the carrier. The enzyme can be adsorbed on a carrier having a hydrophobic surface, and the addition of a hygroscopic substance suppresses agglomeration of the carrier by absorbing excess liquid. The hygroscopic substance may be removed during the removal of volatile components. Contacting of the enzyme-containing liquid and carrier is in a fluidized bed where immobilization and removing volatile components are conducted simultaneously, or contacting is in mixer followed by removing volatile components in a fluidized bed. The enzyme-containing liquid may be atomized onto the carrier in the fluidized bed or in the mixer. Enzymes immobilized include lipase, and the immobili…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.