Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians

US6156569A · kind A · utility

Assignee

• University of Massachusetts Office of Vice Chancellor for Research at Amherst · US

Inventors

• F. Abel Ponce de Leon · Amherst, US
• Catherine Blackwell · Warren, US
• Xiu Gao · Deerfield, US
• James Robl · Granby, US
• Steven Stice · Granby, US
• D. Joseph Jerry · Shutesbury, US

Key dates

• Filing date: Aug 4, 1997
• Grant date: Dec 5, 2000
• Expiry date: Aug 4, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/235
• WIPO field: Other special machines
• WIPO sector: Mechanical engineering

Abstract

A culture system for maintaining avian PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF and SCF. The resultant PGCs are useful for the production of transgenic and chimeric avians, in particular, chickens or turkeys.