Bioartificial extracellular matrix containing hydrogel matrix derivatized with cell adhesive peptide fragment

US6156572A · kind A · utility

Assignee

• Neurotech S.A. · FR

Inventors

• Ravi V. Bellamkonda · Atlanta, US
• John Ranieri · Austin, US
• Patrick Aebischer · Lutry, CH

Key dates

• Filing date: Sep 25, 1998
• Grant date: Dec 5, 2000
• Expiry date: Sep 25, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/813
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A bioartificial extracellular matrix for use in tissue regeneration or replacement is provided by derivatizing a three-dimensional hydrogel matrix with a cell adhesive extracellular matrix protein or cell adhesive peptide fragment of the protein. Preferably, derivatizing is by covalent immobilization of a cell adhesive peptide fragment having the amino acid sequence, ArgGlyAsp, TyrIleGlySerArg or IleLysValAlaVal. Cartilage or tendon can be regenerated by implanting a matrix containing an adhesive peptide fragment that favors chondrocyte invasion. The matrix can be pre-seeded with cells, and tissue can be reconstituted in vitro and then implanted. A cell-seeded matrix can be encapsulated in a semi-permeable membrane to form a bioartificial organ. An agarose hydrogel matrix having an agarose concentration of 0.5-1.25% (w/v) and an average pore radius between 120 nm and 290 nm is preferred. A nerve guidance channel for use in regenerating severed nerve is prepared containing a tubular semi-permeable membrane having openings adapted to receive ends of a severed nerve, and an inner lumen containing the hydrogel matrix having a bound cell adhesive peptide fragment through which the nerve…