DNA strands coding for glycerol-e-phosphate acyltransferase
US6160203A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 1998 |
| Grant date | Dec 12, 2000 |
| Priority date | — |
| Expiry date | Jan 26, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8273
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA strands having the ability to biotechnologically produce glycerol-3-phosphate acyltransferase (ATase) useful for converting the property of the PG of membrane lipids into that of more chilling resistance, specifically a chimeric gene of glycerol-3-phosphate acyltransferase (ATase) cDNA derived from squash in which the about one-third central region (the site cleaved by Kpn I and Hind III) has been replaced with the corresponding region of spinach ATase cDNA, a cDNA derived from squash in which the about one-sixth central region (the site cleaved by Hind III and Sac I) has been replaced with the corresponding region of spinach ATase cDNA, or a chimeric gene of ATase cDNA derived from spinach in which the about one-third 3'-terminal region (the site cleaved by Kpn I and Eco RI) has been replaced with the corresponding region of squash ATase cDNA are disclosed. These chimeric genes can express a chimeric ATase which has a higher substrate selectivity to unsaturated fatty acids. The DNA strand is introduced and expressed in a chilling sensitive plant, so that it can afford a plant the chilling resistance higher than that afforded by the ATase gene derived from a known chilling resi…
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