Process and kit for fragment cloning

US6162633A · kind A · utility

Inventors

• Thomas Metzler · Munich, DE
• Harald Sobek · Penzberg, DE
• Rolf Reichhuber · Penzberg, DE

Key dates

• Filing date: Nov 17, 1998
• Grant date: Dec 19, 2000
• Expiry date: Nov 17, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The subject matter of the invention concerns three cosmid vectors which are suitable for fragment cloning of a size between 7 and 36 kb. These vectors consist of an E. coli ColE1 replica, an ampicillin resistance gene, a multiple cloning cassette, cos sites for in vitro packaging with the lambda packaging extracts as well as fragments from the genome of the bacteriophage lambda. The lambda sequences were selected in a way to prevent lytic processes, vector instabilities (deletions) and unwanted recombination events between lambda DNA and the fragment to be cloned. Depending on the length of the lambda fragment inserted in the corresponding vector heterologous fragments of different lengths can be cloned.