Method for identifying apoptosis modulating compounds
US6165732A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1998 |
| Grant date | Dec 26, 2000 |
| Priority date | — |
| Expiry date | Jul 31, 2018 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for identifying apoptosis-modulating compounds using lipid bilayers are provided. One method involves contacting a compound of interest with a lipid bilayer which contains an ion-channel formed by an anti-apoptotic or pro-apoptotic polypeptide of the BCL-2 family and assaying for changes in the ion conductance properties of the channel, including ion selectivity, single channel conductance and rectification. A second method identifies compounds which can form ion channels in planar lipid bilayers and determines the ion selectivity and pH dependence of such channel formation, where apoptosis modulating activity is predicted based on comparing these channel forming characteristics with those of Bcl-2 family members.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.