Patent · US Expired

In-situ method of analyzing cells

US6165734A · kind A · utility

131Cited by
2References
8Claims
0Family size

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Key dates

Filing dateNov 20, 1998
Grant dateDec 26, 2000
Priority date
Expiry dateNov 20, 2018

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/968
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.