Method and device for flow-cytometric microorganism analysis
US6165740A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 29, 1999 |
| Grant date | Dec 26, 2000 |
| Priority date | — |
| Expiry date | Sep 29, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/911
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
To reduce the effects of the contaminants in the measurement of microorganisms and the reduction of the time necessary for the measurement. Measurement is performed of the microorganism prior to and following culture, and the difference between the two is found. This prevents errors caused by the effect of contaminants contained in the specimens. Since the measurement of the microorganism is performed by means of a flow cytometer, the microorganisms can be measured even when the culture period is short. Moreover, the measurements are accurate, since the contaminants are not measured. Furthermore, the growth form of the microorganisms can be determined by measuring the changes in the intensity of the light emission over the duration of emission of the forward scattered light detected by means of a flow cytometer. Accordingly, based on differences in the particle-size distribution prior to and following culture, it is possible to formulate five major bacterial classifications: Bacilli, Staphylococci, Streptobacilli, Streptococci and yeast fungi.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.