Recombinant production of immunoglobulin-like domains in prokaryotic cells
US6165745A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 17, 1994 |
| Grant date | Dec 26, 2000 |
| Priority date | — |
| Expiry date | Nov 17, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed are recombinant vectors encoding immunoglobulin-like domains and portions thereof, such as T-cell variable domains, antibody Fc-hinge fragments, subfragments and mutant domains with reduced biological half lives. Methods of producing large quantities of such domains, heterodimers, and fusion proteins following expression and secretion by prokaryotic host cells are also reported. Described are single chain T-cell receptors, which are folded into .beta.-pleated sheet structures similar to those of immunoglobulin variable domains; antibody Fc and Fc-hinge domains, which have the same in vivo stability as intact antibodies; and domains engineered to have reduced half lives.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.