Modified-affinity streptavidin
US6165750A · kind A · utility
9Cited by
8References
15Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 5, 1998 |
| Grant date | Dec 26, 2000 |
| Priority date | — |
| Expiry date | Mar 5, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Streptavidin tetramers have at least one monomer containing an amino acid modification that produces a reduced binding affinity for biotin, a modified off-rate, a modified on-rate, or an altered binding enthalpy. Polynucleotides encoding the modified monomers are also provided. The modified streptavidin and chimeric streptavidin molecules are useful in methods of bioseparations and cell sorting, imaging, drug delivery, and diagnostics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.