Limulus reaction-activating substance, method for inactivating the substance and measuring its amount, and method for measuring limulus reaction
US6168924A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 1997 |
| Grant date | Jan 2, 2001 |
| Priority date | — |
| Expiry date | Oct 21, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The presence of endotoxin in the outer membrane of cell wall of a Gram-negative bacteria is used to determine bacterial contamination in a biological product. The amount of endotoxin present in a biological product is accurately measured without influence of a limulus reaction-activating substance which causes a false-positive reaction, by a method including the steps of: inactivating the limulus reaction-activating substance, if any, in the biological product, by exposing the biological product to a surfactant at a temperature ranging from the surfactant's freezing point to 50.degree. C.; and measuring the amount of endotoxin present in the biological product, using a limulus reagent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.