Insertion sequence from a virulent isolate of Burkholderia cepacia, and diagnostic and identification procedures based thereon
US6171789A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 1998 |
| Grant date | Jan 9, 2001 |
| Priority date | — |
| Expiry date | Apr 24, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel IS (insertion sequence) element characteristic of a virulent isolate (ET12/cblA isolate) of Burkholderia cepacia. The IS element is a hybrid of two other IS elements commonly found in isolates of B. cepacia, namely IS402 [SEQ ID NO:2] and IS1356 [SEQ ID NO:3]. The IS hybrid IS element has the sequence shown in the Figure [SEQ ID NO:1]. The fact that the hybrid IS element is characteristic of the virulent isolate means that it can be used as an indicator of that isolate. Thus, the invention includes a method of testing for an ET12/cblA isolate of B. cepacia by testing a sample for the presence of the hybrid IS element, eg. by amplification of the IS element by PCR and identification of the amplified sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.