Enzyme catalysis in organic solutions containing water
US6171813A · kind A · utility
1Cited by
3References
20Claims
0Family size
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Key dates
| Filing date | Oct 16, 1997 |
| Grant date | Jan 9, 2001 |
| Priority date | — |
| Expiry date | Oct 16, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is presented for catalyzing the conversion of substrate into product in an organic reaction solvent with an enzyme-surfactant ion pair. The enzyme-surfactant ion pair comprises the enzyme catalyzing the reaction and a surfactant capable of forming an ion pair with the enzyme. Water present in the organic solvent at a concentration of about 0.03% to about 2.5% is sufficient to enhance the rate of catalysis and stabilize the enzyme without substantially increasing the rate of hydrolysis when compared to the anhydrous enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.