Monitoring amplification of DNA during PCR
US6174670A · kind A · utility
519Cited by
55References
107Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 4, 1997 |
| Grant date | Jan 16, 2001 |
| Priority date | — |
| Expiry date | Jun 4, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.