Method of making biochips and the biochips resulting therefrom
US6174683A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 26, 1999 |
| Grant date | Jan 16, 2001 |
| Priority date | — |
| Expiry date | Apr 26, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/545
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for preparing a biochip are provided herein wherein the biomolecular probe to be used with the biochip is alternatively bound to a hydrogel prepolymer prior to or simultaneously with polymerization of the prepolymer. In particularly preferred embodiments, a polyurethane-based hydrogel prepolymer is derivatized with an organic solvent soluble biomolecule, such as a peptide nucleic acid probe in aprotic, organic solvent. Following derivatization of the prepolymer, an aqueous solution, for example sodium bicarbonate, preferably buffered to a pH of about 7.2 to about 9.5, is added to the derivatized prepolymer solution to initiate polymerization of the hydrogel. Alternatively, a water soluble biomolecule, such as DNA or other oligonucleotide, is prepared in an aqueous solution and added to the polyurethane-based hydrogel prepolymer such that derivatization and polymerization occur, essentially, simultaneously. While the hydrogel is polymerizing, it is microspotted onto a solid substrate, preferably a silanated glass substrate, to which the hydrogel microdroplet becomes covalently bound. Most preferably the hydrogel microdroplets are at least about 30 .mu.m thick, for example ab…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.