Enhanced aggregate removal from bulk biologicals using ion exchange chromatography
US6177548A · kind A · utility
10Cited by
1References
5Claims
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Key dates
| Filing date | Oct 13, 1998 |
| Grant date | Jan 23, 2001 |
| Priority date | — |
| Expiry date | Oct 13, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/065
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods and processes for removing aggregates and other impurities from partially purified protein bulk product, in particular, from partially purified monoclonal antibody, using anion exchange chromatography. In such methods and processes, the pH of the sample is adjusted to be lower than the isoelectric point of the product, preferably by 0.2 logs, and the sample is passed through an ion exchange matrix which is equilibrated to bind the aggregates and host cell DNA as well as endotoxins in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.