Industrial scale process for the preparation of 2-hydroxy-4-methylbutyric acid using a nitrilase
US6180359A · kind A · utility
4Cited by
4References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 24, 1997 |
| Grant date | Jan 30, 2001 |
| Priority date | — |
| Expiry date | Oct 24, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a process for the preparation of 2-hydroxy-4-methylthiobutyric acid or the ammonium salt of 2-hydroxy-4-methylthiobutyric acid by enzymatic hydrolysis of 2-hydroxy-4-methylthiobutyronitrile, comprising: PA1 a) preparing a biological material having a nitrilase activity; PA1 b) immobilizing the biological material, PA1 c) exposing the 2-hydroxy-4-methylthiobutyronitrile to the biological material thus immobilized to obtain the ammonium salt of 2-hydroxy-4-methylthiobutyric acid; and PA1 d) optionally converting the salt obtained to the corresponding acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.