Recoding of DNA sequences permitting expression in yeast and obtained transformed yeast
US6180363A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 23, 1998 |
| Grant date | Jan 30, 2001 |
| Priority date | — |
| Expiry date | Sep 23, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a DNA sequence which encodes a protein of interest which contains regions having a high content of codons which are poorly suited to yeasts, characterized in that a sufficient number of codons which are poorly suited to yeasts is replaced with corresponding codons which are well-suited to yeasts in the said regions having a high content of codons which are poorly suited to yeasts. The present invention relates, more specifically, to DNA sequences which originate from dicotyledonous or monocotyledonous plants, in particular plants of the graminae family which are selected, in particular, from among wheat, barley, oats, rice, maize, sorghum and cane sugar. The present invention also relates to transformed yeasts which contain a DNA sequence according to the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.