Patent · US Expired

Process for the separation of proteins using a CA++ containing eluant

US6180757A · kind A · utility

16Cited by
3References
21Claims
0Family size

Assignee

Inventor

Key dates

Filing dateApr 13, 1999
Grant dateJan 30, 2001
Priority date
Expiry dateApr 13, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K1/20
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention concerns a process of chromatographically separating glycosylated proteins from non-glycosylated proteins by subjecting a solution comprising glycosylated and non-glycosylated proteins to chromatography using a Ca.sup.++ containing eluant. By using this process a fraction comprising non-glycosylated proteins substantially free from glycosylated proteins is obtained. The process may be applied to the separation of proteins used in the medical industry, such as insulin.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.