Process for the separation of proteins using a CA++ containing eluant
US6180757A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 13, 1999 |
| Grant date | Jan 30, 2001 |
| Priority date | — |
| Expiry date | Apr 13, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention concerns a process of chromatographically separating glycosylated proteins from non-glycosylated proteins by subjecting a solution comprising glycosylated and non-glycosylated proteins to chromatography using a Ca.sup.++ containing eluant. By using this process a fraction comprising non-glycosylated proteins substantially free from glycosylated proteins is obtained. The process may be applied to the separation of proteins used in the medical industry, such as insulin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.