Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof
US6180774A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 5, 1997 |
| Grant date | Jan 30, 2001 |
| Priority date | — |
| Expiry date | Aug 5, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided. Also disclosed are novel structural genes which encode insecticidal proteins of B. t. k. and monocotyledonous (e.g. maize) plants containing such novel structural genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.