Patent · US Expired

Vibrio vulnificus molecular probes, antibodies, and proteins

US6183973A · kind A · utility

1Cited by
3References
12Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 4, 1998
Grant dateFeb 6, 2001
Priority date
Expiry dateDec 4, 2018

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K14/28
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention is directed to oligonucleotides used as amplification primers and assay probes for specific and sensitive for virulent strains of V. vulnificus. The target sequence of the probes and primers according to present invention is a capsular polysaccharide (CPS) transport gene (wza) of V. vulnificus. These probes can detect wza DNA or RNA in an unknown sample suspected to have pathogenic strains of V. vulnificus including human, animal, or environmental samples. The invention is also directed to in vitro-expressed protein from the cloned wza for production of polyclonal or monoclonal antibody that is specific for the wza gene product and will detect the V. vulnificus Wza protein in a sample comprising unknown protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.