Method for mass production of antimicrobial peptide
US6183992A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 10, 1999 |
| Grant date | Feb 6, 2001 |
| Priority date | — |
| Expiry date | Mar 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/23
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and antimicrobial peptide, and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of the host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.