Procedure for the detection of high virus concentrations in blood plasma and/or blood serum by means of the polymerase chain reaction
US6183999A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1998 |
| Grant date | Feb 6, 2001 |
| Priority date | — |
| Expiry date | Nov 24, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/701
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A procedure for the detection of high virus concentrations in blood plasma and/or blood serum by means of the polymerase chain reaction (PCR) is described, in which the sensitivity of the PCR is restricted by the use of suboptimal nucleic acid extraction, amplification or detection conditions. This procedure is suitable, for example, for the detection of parvovirus DNA in plasma or serum, it being possible to adjust the sensitivity of the PCR such that the parvovirus DNA is only detected in samples whose DNA content is greater than 10.sup.6 to 10.sup.7 genome equivalents/ml. The detection of the parvovirus DNA amplification carried out is carried out in the sample by measurements of the fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.