Methods for isolation and activation of, and control of differentiation from, skeletal muscle stem or progenitor cells
US6184035A · kind A · utility
71Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 18, 1998 |
| Grant date | Feb 6, 2001 |
| Priority date | — |
| Expiry date | Nov 18, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2503/02
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a method of isolating, maintaining, and/or enriching for stem or progenitor cells derived from diverse organ or tissue sources. The invention specifically teaches that these can be accomplished by the controlled use of subatmospheric oxygen culture, and that the precise oxygen level or levels must be determined empirically and/or by reference to physiologic levels within intact functioning organ or tissue. In particular, culturing skeletal muscle progenitor cells in less than 12% oxygen conditions or under 1% oxygen level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.