Methods for rapid cloning for full length cDNAs using a pooling strategy
US6187544A · kind A · utility
4Cited by
1References
13Claims
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Key dates
| Filing date | Nov 3, 1999 |
| Grant date | Feb 13, 2001 |
| Priority date | — |
| Expiry date | Nov 3, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for high throughput cloning of full length cDNA sequences using a pooling strategy. This method uses a plurality of clone arrays prepared from cDNA libraries which have been preferably enriched for 5 ' mRNA sequences and size fractionated into several discrete ranges (sub-libraries). These arrays are used to rapidly identify the full length cDNA sequence for a DNA segment of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.