Invertebrate intestinal mucin cDNA and related products and methods
US6187558A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 24, 1998 |
| Grant date | Feb 13, 2001 |
| Priority date | — |
| Expiry date | Jun 24, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention represents the disclosure of a novel insect intestinal mucin comprising two nearly identical isoforms, IIM14 and IIM22 respectively. These isoforms of the IIM protein have been identified and cloned using T. ni larva. The cDNA and amino acid sequences have been determined and are disclosed. Both IIM isoforms have an approximate molecular mass of 400 kDa. These sequences once disclosed are useful for the production of transgenic or recombinant vectors including viral, microorganism cell, plant, or animals, wherein the virus, microorganism, cell, plant, or animal is the product of an insertion of a gene expression vector including a DNA that encodes an IIM protein sequence. Thereafter the engineered host of the IIM DNA sequence is capable of expressing said IIM protein in a functional form. Also useful is a purified and isolated recombinant DNA sequence comprising a DNA sequence that codes for an IIM protein. The recombinant DNA sequence used can be a cDNA sequence for either IIM isoform IIM14 or IIM22, SEQ. ID.'s No. 1; and 3 respectively. The current invention also provides for the use of the purified amino acid sequences of IIM isoforms IIM14 or IIM22, SEQ. ID.'s 2 o…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.