Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA

US6187575A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Harald Sobek · Penzberg, DE
• Manfred Schmidt · Krefeld, DE
• Bruno Frey · Penzberg, DE
• Klaus Kaluza · Bad Heilbrunn, DE

Key dates

• Filing date: Aug 6, 1998
• Grant date: Feb 13, 2001
• Expiry date: Aug 6, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/2497
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.