Vectorial cloning system of DNA

US6187589A · kind A · utility

Assignee

• November AG Novus Medicatus Bertling Gesellschaft fur Molekulare Medizin · DE

Inventor

• Wolf Bertling · Erlangen, DE

Key dates

• Filing date: Oct 5, 1999
• Grant date: Feb 13, 2001
• Expiry date: Oct 5, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a vectorial cloning system consisting of a sequence of nucleotides, containing a fusion sequence and one or several restriction endonucleases, recognition sites for restriction endonucleases cutting outside their recognition sites, in addition to containing one or several other restriction endonuclease recognition sites which can be used to clone a foreign protein as well as the sequence for the desired foreign protein, wherein the foreign protein sequence is directly located on the fusion sequence after a subsequent restriction with the restriction endonucleases, followed by religation.