Patent · US Expired

Efficient enrichment and detection of disseminated tumor cells

US6190870A · kind A · utility

46Cited by

5References

12Claims

0Family size

Assignees

Amcell Corporation · US
Miltenyi Biotec, Inc. · DE

Inventors

Jürgen Schmitz · Dormagen, DE
Stefan Miltenyi · Wittlich, DE
Fawn Qian · Sunnyvale, US
Aaron Kantor · Redwood City, US

Key dates

Filing date	Jun 21, 1996
Grant date	Feb 20, 2001
Priority date	—
Expiry date	Jun 21, 2016

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S436/825
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Tumor cells, particularly carcinoma cells, are separated from peripheral blood by magnetic sorting. The tumor cells are magnetically labeled with antibodies directed to tissue specific antigens, preferably cytoplasmic proteins. Labeling for cytoplasmic antigens is accomplished first permeabilizing, then fixing the cells. The cells are separated on a magnetic matrix. The number of tumor cells in the enriched fraction is used to calculate the number of tumor cells present in a patient hematopoietic sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.