Expression of an exogenous gene in a mammalian cell by use of a non-mammalian DNA virus having an altered coat protein
US6190887A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Feb 28, 2000 |
| Grant date | Feb 20, 2001 |
| Priority date | — |
| Expiry date | Feb 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2810/6081
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Disclosed are methods, nucleic acids, and cells for expressing an exogenous gene in a mammalian cell, involving introducing into the cell a non-mammalian DNA virus (e.g., a baculovirus) having an altered coat protein, the genome of which virus carries an exogenous gene, and growing the cell under conditions such that the gene is expressed. Also disclosed are methods for treating gene deficiency disorders, neurological disorders, or cancers in a mammal by (1) providing to a cell a therapeutically effective amount of a non-mammalian DNA virus having an altered coat protein, the genome of which virus carries an exogenous, therapeutic gene and (2) growing the cell under conditions such that the exogenous gene is expressed in the mammal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.