Microbial production of indigo
US6190892A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 1995 |
| Grant date | Feb 20, 2001 |
| Priority date | — |
| Expiry date | Nov 20, 2015 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH04W84/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is provided an improved process for the biosynthetic production of indigo, the improvement comprising removing unwanted by-products such as isatin or indirubin from the broth in which such indigo is produced. Isatin can be removed by enzymatic activity using an isatin-removing enzyme such as an isatin hydrolase, or by other techniques such as process parameters (elevated temperature, pH), or by contacting the broth containing the isatin with appropriate adsorption compounds/compositions such as carbon or appropriate resins. Since isatin is the precursor of indirubin, the indirubin levels are decreased as a result of isatin removal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.