Cloning and producing the N.BstNBI nicking endonuclease
US6191267A · kind A · utility
40Cited by
2References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 2, 2000 |
| Grant date | Feb 20, 2001 |
| Priority date | — |
| Expiry date | Jun 2, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.