Inhibition of picornavirus genome replication by interference with VPg-nucleotidylylation and elongation
US6194141A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 31, 1999 |
| Grant date | Feb 27, 2001 |
| Priority date | — |
| Expiry date | Mar 31, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods of inhibiting picornavirus genome replication in a subject. In particular, methods for interfering with VPg uridylylation and elongation are provided. The methods comprise administering to a subject an effective amount of at least one of VPg, VPg analog, VPg homology or biologically active fragment thereof as well as oligonucleotides, divalent cations, ribonucleotide or deoxyribonucleotide. Also provided are methods of identifying an inhibitor of picornaviral replication which comprise adding a potential inhibitor of picornaviral replication to an in vitro assay and analyzing levels of VPg uridylylation reaction products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.