Recombinant expression of proteins from secretory cell lines
US6194176A · kind A · utility
8Cited by
17References
59Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Jan 17, 1997 |
| Grant date | Feb 27, 2001 |
| Priority date | — |
| Expiry date | Jan 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.