Electrochemical denaturation and annealing of nucleic acid
US6197508A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 31, 1998 |
| Grant date | Mar 6, 2001 |
| Priority date | — |
| Expiry date | Jul 31, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process disclosed is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process may also employ a promoter compound such as methyl viologen to speed denaturation. The process may be used in the detection of nucleic acid by hybridizing with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction. A process is described for annealing complementary strands of nucleic acid by application of an electrical voltage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.