Metabolic controlled fermentation procedure for the manufacture of lovastatin hydroxy acid
US6197560A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 17, 1999 |
| Grant date | Mar 6, 2001 |
| Priority date | — |
| Expiry date | Mar 17, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P17/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing lovastatin by a microorganism in a fermentation process having a seed culture stage and a main fermentation stage, including PA0 a) cultivating a microorganism biomass in the seed culture stage to produce an inoculum; PA0 b) transferring the inoculum into a fermentation medium in the main fermentation stage; and, PA0 c) maintaining steady stage conditions in the main fermentation stage, thereby producing a fermentation broth containing lovastatin. Preferably, the steady state conditions are maintained in the main fermentation stage by one or more of feeding of organic carbon sources; controlling glucose and/or total reducing sugar content; feeding of organic and/or inorganic nitrogen sources; controlling pH; controlling foam level; controlling the mass of the fermentation broth by withdrawals and feedings; and, controlling the dissolved oxygen level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.