Cold-active protease CP70
US6200793A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jul 27, 1998 |
| Grant date | Mar 13, 2001 |
| Priority date | — |
| Expiry date | Jul 27, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them, and the substrate specificity decreases in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable in the range of pH 6.5 to pH 10.0 at 30.degree. C. for 1 hour; (d) optimal temperature: about 40.degree. C.; (e) temperature stability: at pH 7 for 1 hour, the protease is active at a temperature up to 30.degree. C., but it is inactivated at 40.degree. C. as much as about 40% and completely inactivated at 50.degree. C. in about 10 minutes; (f) enzyme activity: the protease has 50% or more of its maximum activity at about 20.degree. C.; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.