Method for detecting reactions by means of coincidence analysis

US6200818A · kind A · utility

Assignee

• EVOTEC BIOSYSTEMS GMBH · DE

Inventors

• Manfred Eigen · Göttingen, DE
• Thorsten Winkler · Hamburg, DE
• Jens Stephan · Hamburg, DE
• Petra Schwille · Dresden, DE
• Andre Koltermann · Icking, DE
• Ulrich Kettling
• Klaus Dorre · Hamburg, DE
• Jan Bieschke · Oettingen, DE

Key dates

• Filing date: Jun 22, 2000
• Grant date: Mar 13, 2001
• Expiry date: Jun 22, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6421
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method for detecting reactions and conformational changes of analytes in a sample by coincidence analysis, as follows: the sample is marked with at least two different fluorescent dyes and then illuminated with at least one laser in order to stimulate the emission of fluorescence; the fluorescence signals are detected by at least two detection units and each signal is broken down into any simultaneous time segments with the desired time slot widths; the number of signals contained in at least one time segment and/or the time intervals between signals in the time segments are detected; a coincidence analysis of the data detected is carried out for at least one time segment of the first detection unit with at least one isochronous time segment of the second detection unit; statistics are produced showing the results of the coincidence analysis and/or the results are subjected to a threshold value analysis, and these statistics or a combination of more statistics are evaluated for the presence of characteristic features.