Biospecific assay method
US6204068A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 1997 |
| Grant date | Mar 20, 2001 |
| Priority date | — |
| Expiry date | May 13, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/807
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention is thus related to a biospecific multiparameter assay employing single molecule detection. In particular, this invention is related to a new method for determination of several different biomolecules simultaneously in the same reaction solution. The method uses a fluorescent label for labelling biospecific primary probes and a combination of other labels, bound to secondary probes. Complexes are formed comprising primary probes, analyte molecules and secondary probes in the biospecific reaction. These single molecule complexes are counted selectively while discriminating the signals from other fluorescent molecules using confocal fluorometry or two-photon excitation fluorometry including an auto- and cross-correlator for the signals obtained from said fluorescent labels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.