Diagnostics based on mass spectrometric detection of translated target polypeptides
US6207370A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 2, 1997 |
| Grant date | Mar 27, 2001 |
| Priority date | — |
| Expiry date | Sep 2, 2017 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a means of detecting and identifying mutations in a genetic region, and a means of quantifying the number of repeat units in, for example, a trinucleotide repeat, by transcription/translation of the genetic region into a target polypeptide. The method requires neither radioisotopic nor fluorescent labeling of the target polypeptide. In particular, the invention is based on mass spectrometric determination of the mass of the encoded target polypeptide and comparison of the mass of the polypeptide with its own expected mass or with the mass of a polypeptide of known identity. Depending on the target polypeptide to be identified, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.