Systematic identification of essential genes by in vitro transposon mutagenesis

US6207384A · kind A · utility

Assignees

• The General Hospital Corporation · US
• Trustees of Tufts College · US
• President and Fellows of Harvard College · US

Inventors

• John J. Mekalanos · Cambridge, US
• Brian Akerley · Brookline, US
• Eric Rubin · Newton, US
• Andrew Camilli · Sharon, US

Key dates

• Filing date: Mar 26, 1999
• Grant date: Mar 27, 2001
• Expiry date: Mar 26, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6811
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention features a general system for the identification of essential genes in organisms. This system is applicable to the discovery of novel target genes for antimicrobial compounds, as well as to the discovery of genes that enhance cell growth or viability.