Bidirectional PCR amplification of specific alleles
US6207425A · kind A · utility
7Cited by
3References
13Claims
0Family size
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Key dates
| Filing date | Sep 10, 1998 |
| Grant date | Mar 27, 2001 |
| Priority date | — |
| Expiry date | Sep 10, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6858
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Bi-directional polymerase chain reaction (PCR) amplification of specific alleles (Bi-PASA). Two outer primers (P and Q) and two inner primers (A and B) are used. A and B are each specific for different alleles. In heterozygotes, three segments are amplified: a segment of size AQ resulting from one allele, another segment of size PB resulting from the second allele, and a combined segment of size PQ. In homozygotes, segment PQ and either segments AQ or PB amplify.
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