Ribozyme-mediated synthesis of circular RNA

US6210931A · kind A · utility

Assignee

• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• Paul A. Feldstein · Sacramento, US
• Robert A. Owens · Amherst, US
• Laurene Levy · Beltsville, US
• John W. Randles · Stirling, AU

Key dates

• Filing date: Nov 30, 1998
• Grant date: Apr 3, 2001
• Expiry date: Nov 30, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/122
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Circular RNAs may be synthesized by inserting DNA fragments into a plasmid containing sequences having the capability of spontaneous cleavage and self-circularization. Insertion of the DNA fragments allows RNAs of predetermined size to be constructed. In addition, a two-dimensional polyacrylamide gel electrophoresis system having a second dimension more highly cross-linked than the first dimension permits the separation and analysis as well as the precise sizing of both linear and circular RNAs produced by the synthetic method.