Expression and secretion of heterologous polypeptides from caulobacter

US6210948A · kind A · utility

Assignee

• University of British Columbia · CA

Inventors

• John Smit · Richmond, CA
• Wade H. Bingle · Vancouver, CA
• John F. Nomellini · Richmond, CA

Key dates

• Filing date: Mar 30, 1999
• Grant date: Apr 3, 2001
• Expiry date: Mar 30, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/20022
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

DNA constructs are provided which code for a chimeric protein in which the C-terminal region corresponds to the extreme C-terminal amino acids of a Caulobacter S-layer protein, fused with a heterologous polypeptide. Bacterial cells containing the DNA constructs, or which express the DNA constructs and secrete the resulting protein, are provided. Chimeric proteins including the C-terminal amino acids of a Caulobacter S-layer protein are provided, including proteins which include antigenic epitopes of the Infectious Hematopoietic Necrosis Virus.