Process for determining a bindable analyte via immune precipitation and reagent therefor

US6210975A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Johann Karl · Peißenberg, DE
• Fridl Lang · Tutzing, DE

Key dates

• Filing date: Oct 4, 1991
• Grant date: Apr 3, 2001
• Expiry date: Oct 4, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/824
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention involves methods for determining analytes, and reagents for use in these methods. The methods and reagents use one or both of a polyvinyl/pyrolidone with a molecular weight of at least 360,000, and a polyethylene glycol with molecular weight of at least 40,000. The assays are carried out nephelometrically, or turbidometrically. The reagents include at least one antibody which binds the analyte. The hook effect is reduced or avoided in the practice of the invention.