Enzyme catalysis in carbon dioxide fluids
US6211422A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jul 13, 1999 |
| Grant date | Apr 3, 2001 |
| Priority date | — |
| Expiry date | Jul 13, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the enzymatic production of a desired product in a liquid or supercritical carbon dioxide fluid. The method comprises: (a) providing a reaction mixture comprising at least one reaction substrate and a crosslinked enzyme crystal catalyst in a liquid or supercritical carbon dioxide fluid; and (b) reacting said substrate with said crosslinked enzyme crystal catalyst in said carbon dioxide fluid to produce said product. The reacting step is typically followed by the step of (c) separating the product from said reaction mixture. The crosslinked enzyme crystal catalyst preferably comprises an enzyme crystal crosslinked with a multifunctional crosslinking agent, said crosslinked enzyme crystal having resistance to exogeneous proteolysis, such that the crosslinked enzyme crystal retains at least about 80 percent of its initial activity after incubation for three hours in the presence of a concentration of Pronase.TM. protease that causes the soluble uncrosslinked form of the enzyme that is crystallized to form said enzyme crystal that is crosslinked to lose at least about 80 percent of its initial activity under the same conditions. Reaction mixtures useful for carrying out th…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.