Peptide production as fusion protein in transgenic mammal milk
US6211427A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 8, 1996 |
| Grant date | Apr 3, 2001 |
| Priority date | — |
| Expiry date | Oct 8, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Peptides can be produced in and purified from the milk of transgenic animals. The peptides are made as fusion proteins with a suitable fusion partner such as .alpha.-lactalbumin, which is a natural milk protein. The fusion partner protein acts to promote secretion of the peptides and, at least in the case of .alpha.-lactalbumin, allows a single-step purification based on specific affinity. The peptide is released from the purified fusion protein by a simple cleavage step and purified away from the now liberated .alpha.-lactalbumin by repeating the same affinity purification method. A particular advantage of producing peptides via this route, in addition to the obvious advantages of high yield and biocompatibility, is that specific post-translational modifications, such as carboxy terminal amidation, can be performed in the mammary gland.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.