Monoclonal antibodies of isotype IgG which bind specifically to glutamate decarboxylase, and uses thereof

US6214568A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Josef Endl · Weilheim, DE
• Michael Brandt · Iffeldorf, DE
• Herbert Jungfer · Starnberg, DE
• Winfried Albert · Eberfing, DE
• Rosemarie Kientsch-Engel · Feldafing, DE
• Werner Scherbaum · Ulm, DE
• Wiltrud Richter · Ulm, DE
• Thomas Eiermann · Ulm, DE

Key dates

• Filing date: Jul 29, 1998
• Grant date: Apr 10, 2001
• Expiry date: Jul 29, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/21
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns human monoclonal antibodies of the IgG isotype against human pancreatic islet cells which can be obtained by immortalizing human lymphocytes of prediabetics or diabetics, treating the culture supernatant of the immortalized cells with a conjugate of antibodies against human Fc .gamma. and a label, subsequently treating with human immunoglobulin, incubating with immobilized human pancreatic islet cells identifying an immortalized human cell culture which produces an antibody against pancreatic islet cells via determination of the label bound to the immobilized islet cells, isolating a human immortalized cell which produces this antibody, propagating this immortalized cell and isolating the monoclonal antibody produced by these cells. The invention also concerns a process for the isolation of an islet cell antigen to which such antibodies bind as well as a method for the determination of antibodies against an islet cell antigen of the pancreas.